TriM Scope 2photon microscope

Profile: Two photon microscope with am OPO that extends the wavelength range. Single beam scannming with PMT detection or multibeam scanning with CCD detection. SHG detection possible. A time domain FLIM detector is available.

LaVision Biotec TriM Scope multi photon microscope on a Nikon Eclipse FN-1 upright microscope (the FN-1 microscope is hidden in the big white box for light protection).

LaVision TriM-Scope on Nikon FN-1 stand

The system is equipped with a mode locked tunable Ti:Sapphire laser (80 MHz, 730 - 1050 nm) as well as an optical parametric oscillator (OPO) that extends the wavelength range from 1100 - 1300 nm making red dyes accessible to the scope. The TriM Scope is able to split up the incoming laser beam in 2, 4, 8, 16, 32 or 64 beamlets for fast imaging with 2 CCD cameras (one with an automated filter wheel). Alternatively imaging can be done with a single beam and detection by 4 PMTs (blue, green, red and transmission) located directly after the objective, a mode that is ultra sensitive and allows for deep sample penetration. A time correlated single photon counting (TCSPC) detector for fluorescence life time imaging microscopy (FLIM) is also available. The main objectives are 2 long working distance (LWD) dipping lenses (16x NA 0.8 and 60x NA 1.0). A intravital stage with 4 cm vertical clearance and ± 2.5 cm movement in the x/y axes allows measurements in whole animals. The TriM Scope may only be used for projects that are necessarily requiring a multi photon microscope and will always be operated by Nico Dross from the NIC staff.

 

Bearbeiter: Christian Ackermann

Latest Revision: 2013-10-09

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