Nikon Ti-HCS microscope
Profile: High content screening system for multiwell plates in phase contrast and/or fluorescence.
Here we have a fully automated, inverted Nikon Ti2 microscope made for high content screening tasks. The microscope is encased by a evironmental box from Oko Lab for temperature-, CO2- and humidity control. By a software module names "JOBS" in the Nikon NIS-Elements acquisition software it is possible to screen in x/y, z and t everything from a LabTek dish to a multiwell plate. Images can be taken in brightfield and epifluorescence.
- Nikon Plan Fluor 10x NA 0.3 phase contrast (working distance 16mm; corrected for 0.17mm coverslips (= type #1.5))
- Nikon S Plan Fluor ELWD 40x NA 0.60 (working distance 3.6 - 2.8mm; adjustable for glas and plastic bottom dishes with a thickness between 0 and 2mm).
- Nikon Plan Fluor 20x NA 0.75 Multi Immersion (working distance 0.35mm; water, glycerol. oil; adjustable for glas bottom dishes of type #1 - 2)
- Nikon Plan Apo VC 20x NA 0.75 (working distance 1.0 mm; corrected for 0.17mm coverslips (= type #1.5))
Nikon DS-Qi2 high-sensitive CMOS monochrome camera with 2400 x 2400 pixel resolution (in total 4908×3264, the image is cropped to fit the FOV of the microscope) and a quantum efficiency of 80% at 500nm.
Fluorescent light source
Lumencor Sola SE II
Bearbeiter: Christian Ackermann
Latest Revision: 2017-05-17