Nikon AZ100 zoom microscope

Profile: Microscope especially made for imaging of larger specimens with epifluorescence or DIC contrast. Uses low magnifying, long working distance objectives. A confocal scanhead may also be attached.

The Nikon AZ100 is a multizoom microscope equipped with the following objectives:

AZ Plan Apo 1x NA 0.1 (working distance 35mm)
AZ Plan Fluor 2x NA 0.2 (working distance 45mm)
AZ Plan Fluor 5x NA 0.5 (working distance 15mm)

By an optical zoom mechanism each objective's magnification can be further increased by a factor of up to 8. This results in magnifications ranging from 1x (with objective 1x and zoom-factor 1x) to 40x (with objective 5x and zoom-factor 8x).
All objectives do not require the specimens to be covered with coverslips. In addition the 5x objective has a correction collar to compensate spherical aberration if specimens are submerged in medium.
Apart from Differential Interference Contrast (DIC), the AZ100 offers epifluorescence illumination. Normally the AZ100 is being used with CCD cameras but these may be replaced by the Nikon C2 confocal's scanning head, rendering the AZ100 to a low to moderately magnifying confocal microscope.

Nikon AZ100 Zoom Microscope

Camera:

THe AZ100 is equipped with with a Nikon DS-Ri2 color camera. It uses the same 24 x 36mm CMSO chip as the DS-Qi2 but with Bayer filter. Two resolution settings are possible: 1636 x 1088 pixel or 4908 x 3264 pixel.

Filter Cubes

The AZ100 is equipped with the following filter cubes:

"BV-2A": EX 420/40 DM 455 EM 470 longpass filter
"FITC": EX 480/30 DM 505 EM 535/40
"TexasRed": EX 560/40 DM 595 EM 630/60

EX = excitation wavelength; DM = dichroic mirror (shorter wavelength pass the mirror, longer wavelength are being reflected for detection); EM = emission wavelength. Notation: center wavelength [nm]/bandwidth [nm]

Bearbeiter: Christian Ackermann

Latest Revision: 2015-01-20